EFEKTIVITAS ECO‑ENZIM TERHADAP PENURUNAN JUMLAH BAKTERI PADA SPONS CUCI PIRING MENGGUNAKAN COLONY COUNTER
DOI:
https://doi.org/10.53625/jirk.v5i4.11310Keywords:
eco-enzim, colony counter,, mikroba, spons cuci piringAbstract
Spons cuci piring merupakan salah satu media dengan tingkat kontaminasi mikroba yang tinggi akibat kondisi lembap dan keberadaan sisa bahan organik, sehingga berpotensi menjadi vektor penyebaran bakteri patogen penyebab penyakit. Upaya pengendalian bakteri umumnya menggunakan sabun cuci piring, namun residu surfaktan kimiawi berpotensi menimbulkan dampak negatif terhadap kesehatan dan lingkungan. Eco-enzim, hasil fermentasi limbah organik dengan gula, diketahui mengandung senyawa antimikroba alami sehingga berpotensi sebagai alternatif ramah lingkungan. Penelitian ini bertujuan mengkaji efektivitas eco-enzim dalam menurunkan jumlah koloni bakteri pada spons cuci piring. Penelitian dilaksanakan secara eksperimental di laboratorium menggunakan variasi konsentrasi eco-enzim (0%, 5%, 10%, 15%, 20%). Jumlah koloni bakteri dihitung menggunakan colony counter setelah proses inkubasi. Hasil penelitian menunjukkan adanya hubungan negatif antara konsentrasi eco-enzim dan jumlah koloni bakteri. Konsentrasi 20% terbukti paling efektif menurunkan jumlah koloni menjadi 8,000 x 102 CFU/mL. Analisis FTIR mendeteksi keberadaan asam organik dan enzim aktif yang berperan dalam aktivitas antibakteri. Dengan demikian, eco-enzim berpotensi dikembangkan sebagai bahan pembersih ramah lingkungan sekaligus solusi pengelolaan limbah organik rumah tangga.
References
Ahmed, Z. (2024). Environ mental S cien ce A rchiv es FTIR Analysis of Enzymatically Biopolished Eco-friendly Jute-Cotton Union Fabrics. 2. https://doi.org/10.5281/zenodo.12606235
Ayu SetiyaNingrum, D., Diah Nutfindiani, E., Margaretha, Z., & Rusdiana Surya Efendi, M. (2024). Indonesian Journal of Chemical Research The Implementation of FT-IR Method for Compound Detection in Eco-Enzyme Applied as Hydrogel Patch. J. Chem. Res, 12(1), 55–63. https://doi.org/10.30598//ijcr.2024.12-set
Bambang Widjanarko, S., Putri Aulia, L., Khoirunnisa Departemen Ilmu Pangan dan Bioteknologi -Fakultas Teknologi Pertanian -, Y., & Korespondensi, P. (2023). PROFIL SIFAT FISIKO-KIMIA DAN MIKROBIOLOGI CAIRAN ECO-ENZYME DARI BEBERAPA JENIS BUAH DAN DAUN CEMARA UDANG (Casuarina equisetifolia) Physicochemical and Microbiological Properties of Eco-Enzyme from Several Fruit Waste and Cemara Udang (Casuarina equisetifolia) leaf. In Jurnal Teknologi Pertanian (Vol. 23, Issue 2).
Cupic, V., Bartula, M., Ivanovic, S., Borozan, S., Mujezinovic, I., & Cupic-Miladinovic, D. (2023). Household chemicals as possible causes of poisoning and environmental pollution. Zbornik Matice Srpske Za Prirodne Nauke, 145, 97–121. https://doi.org/10.2298/zmspn2345097c
Hupka, M., Kedia, R., Schauer, R., Shepard, B., Granados-Presa, M., Vande Hei, M., Flores, P., & Zea, L. (2023). Morphology of Penicillium rubens Biofilms Formed in Space. Life, 13(4). https://doi.org/10.3390/life13041001
Jr, A. M. B. (2024). MARVEL SPONGE; A VOGUISH VERSATILE DISHWASHING TOOL [University of Southeastern Philippines]. https://doi.org/10.13140/RG.2.2.21152.19203
Khasanah, Z., Shilfiyah, A. N., & Hidayati, R. N. (2023). PEMANFAATAN ECO-ENZYME BERBAHAN DASAR LIMBAH KULIT JERUK UNTUK PEMBUATAN SABUN CAIR YANG RAMAH LINGKUNGAN SERTA PENGUJIAN ANTIBAKTERI DAN ORGANOLEPTIKNYA. https://www.researchgate.net/publication/391466599
Li, X., Li, D., Yan, J., Zhang, Y., Wang, H., Zhang, J., Ahmed, T., & Li, B. (2021). Effect of Plant-Growth-Promoting Fungi on Eggplant (Solanum melongena L.) in New Reclamation Land. https://doi.org/10.3390/agriculture
Møretrø, T., Ferreira, V. B., Moen, B., Almli, V. L., Teixeira, P., Kasbo, I. M., & Langsrud, S. (2022). Bacterial levels and diversity in kitchen sponges and dishwashing brushes used by consumers. Journal of Applied Microbiology, 133(3), 1378–1391. https://doi.org/10.1111/jam.15621
Møretrø, T., Moen, B., Almli, V. L., Teixeira, P., Ferreira, V. B., Åsli, A. W., Nilsen, C., & Langsrud, S. (2021). Dishwashing sponges and brushes: Consumer practices and bacterial growth and survival. International Journal of Food Microbiology, 337. https://doi.org/10.1016/j.ijfoodmicro.2020.108928
Naveen, H. K. S., Kumar, G., & Rao, B. (2010). Extracellular biosynthesis of silver nanoparticles using the filamentous fungus Penicillium sp. https://www.researchgate.net/publication/209164116
O. N., A.-A., & E. A., C. (2023). Antibiotics and Antifungal Resistance Patterns of Microbial Isolates from Dish Washing Sponges in the University of Port-Harcourt, Nigeria. Asian Journal of Biology, 19(1), 45–52. https://doi.org/10.9734/ajob/2023/v19i1358
Ondrejková, E., Hronská, H., Štefuca, V., Bláhová, M., & Rosenberg, M. (2023). Isolation, Specificity, and Application of β-N-Acetylhexosaminidases from Penicillium crustosum. Applied Sciences (Switzerland), 13(6). https://doi.org/10.3390/app13063399
Rahmathullah, A., Babu, S., & Krishnan, M. (2025). Scrubbing with risk: health and environmental hazards of kitchen cleaning tools and sustainable alternatives. Arashi Journal of Metals and Material Sciences, 2(1), 19–29. https://doi.org/10.54646/jmms.2025.0003
Restuati, M., Wuyung, W., Brata, W., & Pratiwi, N. (2023). PROCESSING TECHNOLOGY OF BUAS-BUAS LEAVES (PREMNA PUBESCENS BLUME) INTO LIQUID SOAP AND HAND SANITIZER TESTED ORGANOLEPTIC AND ANTI-BACTERIAL. In RUSSIAN LAW JOURNAL: Vol. XI. www.DeepL.com/Translator
Sharma, M. V., Sagar, A., & Joshi, M. (2015). Study on Antibacterial Activity of Agaricus bisporus (Lang.) Imbach. In Int.J.Curr.Microbiol.App.Sci (Vol. 4, Issue 2). http://www.ijcmas.com
Siregar, S. S., Herry Hermansyah, & Hendayani. (2023). Uji Kontaminasi Bakteri Spons Pencuci Piring Rumah Makan Berdasarkan Lama Penggunaan. Jurnal Pustaka Medika, 2, 59–63.
Vama, L., & Cherekar, M. N. (2020). PRODUCTION, EXTRACTION AND USES OF ECO-ENZYME USING CITRUS FRUIT WASTE: WEALTH FROM WASTE. In Biotech. Env. Sc (Vol. 22, Issue 2).
